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Ensure technicians are trained in aseptic techniques to avoid cross-contaminating the samples during collection.
Count the visible colonies (Colony Forming Units, or CFU) directly on the plate. Express the result as: Result=X CFU / plateResult equals X CFU / plate
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The standard defines precise protocols for four primary surface sampling tools:
The International Standard ISO 18593 is the global benchmark for microbiology in the food chain, specifically focusing on horizontal methods for sampling techniques from surfaces. For food manufacturers, laboratory technicians, and quality assurance managers, obtaining a verified PDF copy of this standard and understanding its practical implementation is critical to ensuring food safety and regulatory compliance.
By following this guide, laboratories can ensure that they are using a standardized method for the enumeration of microorganisms in food and animal feeding stuffs, and that their results are accurate and reliable. This link or copies made by others cannot be deleted
: To detect and enumerate culturable microorganisms, including pathogenic bacteria (e.g., Listeria monocytogenes ), non-pathogenic bacteria, yeasts, and moulds.
: This is specifically covered by ISO 17604.
Practical considerations for users
Agar-filled plates are pressed directly onto the surface.
Here is a comprehensive guide to ISO 18593, its core methodologies, and how to access verified versions of the documentation. What is ISO 18593?
ISO 18593:2018 details four main, validated sampling techniques for recovering microorganisms from surfaces. The choice of method depends on the surface type, the suspected microorganism, and the lab's resources. Try again later
Samples must be cooled immediately after collection and maintained at a temperature between 1°C and 8°C during transit. Freezing must be strictly avoided, as ice crystals can destroy microbial cells.
